生物

GADPH_木鱼的全国中学生生物奥林匹克竞赛的一些琐事

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme in the glycolytic pathway that catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate, coupled with the reduction of NAD+ to NADH.

Beyond its central role in energy metabolism, GAPDH is renowned for its exceptional kinetic properties, which let Sir R. young give it a name as God of enzyme.

1. High Catalytic Efficiency: GAPDH exhibits a very high turnover number ($k_{cat}$), allowing it to process substrates rapidly to meet the high energy demands of the cell.

2. Near-Diffusion-Limited Specificity: It has a high specificity constant ($rac{k_{cat}}{K_m}$) for its substrate, glyceraldehyde-3-phosphate, meaning it binds and converts the substrate with remarkable efficiency, approaching the theoretical limit of diffusion control.

3. Cooperative Binding: In many organisms, GAPDH functions as a tetramer and displays positive cooperativity for its substrate, ensuring a sharp metabolic response to changes in substrate concentration.

4. Strict Cofactor Specificity: It shows high selectivity for NAD+ over NADP+, which is crucial for maintaining the cytosolic NAD+/NADH balance required for continuous glycolysis.

Due to these robust kinetic features and its abundant, stable expression across tissues, GAPDH is also widely used as a housekeeping gene or protein control in molecular biology experiments.

————木鱼参考《生物化学原理(第三版)》对3-磷酸甘油醛脱氢酶的英文定义与简介

那个名字超长的帖子就作为学习进度加督促自己的帖子吧❤️(○ v ○。)

这个帖子继承的是无题_木鱼的生物竞赛什么都可可可的帖子

各位热爱生命科学正在认真学习生竞并有意识将自己生竞学好的生竞生

恭喜你发现了宝藏^_^

来自可以有@这个可以有(保佑我进省队) 的一些可能帮助到学习的东西

b站 圆梦 公益性组织 有企鹅群

可以去找离心机(客服姐姐)就是说是可以有 拉来的，自己什么水平说清楚，会有大佬指导的

企鹅群 生竞幻想乡 群号Ø 你乡里扔块板砖能砸死一堆国集

河佬发的文件要看，全是精华(前世界第三)

生化不会找CKM

动物不会找5+0

李世元生理很好

河佬的植物很好

行为生态一类宏观不会的可以试试找猫娘姐

遗传找璐姐 目前在东京大学

细胞也找河佬

反正抓不到别人你就在群里问，回复你的大概率是某个国集。

水题要做，幻想乡的水题非常有助于我们记忆一些猎奇的知识。

宇生舍的题目很好

圆梦的梦佬搞的月赛要参加